Pharmcia（GE）葡聚糖凝膠G-15Sephadex G-15
Sephadex G-15 is well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1 500 molecular weight.
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.
Pharmcia（GE)葡聚糖凝膠G-15Sephadex G-15
| Matrix | Cross-linked dextran |
| Particle Size Wet | 60 µm-180 µm |
| Partical Size Dry | 40 µm-120 µm |
| pH Stability Working Range | 2-13 |
| Flow Rate | < Darcy´s Law1) |
| Pressure/Flow Specification | 20-47 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d. |
| Fractionation [Mr] Globular Proteins | 1.5 x 103 |
| Fractionation [Mr] Dextrans | >< 1.5 x 103 |
| Storage Conditions | 4 to 30°C |
| Autoclavable | In wet form (pH 7.0) at 120°C for 30 min |
| 1)The beads behave as rigid spheres obeying Darcy's Law. | |
| Handbook |
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| Size Exclusion Chromatography: Principles and Methods Summary This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close |
| Magazine |
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| Upstream Downstream issue 2 Close |
| Material Safety Data Sheet |
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| Sephadex™ G-15, 100 g Close |
| Sephadex™ G-15, 100 g Close |
| Selection guide |
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| Protein and Peptide Purification: Technique selection guide Summary This technique selection guide is designed to help you in selecting the technique most suited to your application. It covers ion exchange chromatography (IEX), reversed phase chromatography (RPC), hydrophobic interaction chromatography (HIC), gel filtration (GF), and affinity chromatography (AC). Close |
Pharmcia（GE)葡聚糖凝膠G-15Sephadex G-15 詳細產(chǎn)品信息可和選購
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